Our faculty research broadens the knowledge of kidney disease.

Open Microfluidic Coculture Reveals Paracrine Signaling From Human Kidney Epithelial Cells Promotes Kidney Specificity of Endothelial Cells

Tianzi Zhang, Daniel Lih, Ryan J Nagao, Jun Xue, Erwin Berthier, Jonathan Himmelfarb, Ying Zheng, Ashleigh B Theberge

We designed an open microfluidic platform to study the role of human kidney tubular epithelial cells in supporting EC specificity. The platform consists of two independent cell culture regions segregated with a half wall; culture media is added to connect the two culture regions at a desired timepoint, and signaling molecules can travel across the half wall (paracrine signaling).

Feature-rich covalent stains for super-resolution and cleared tissue fluorescence microscopy

Chenyi Mao, Min Yen Lee, Jing-Ru Jhan, Aaron R. Halpern, Marcus A. Woodworth, Adam K. Glaser, Tyler J. Chozinski, Leonard Shin, Jeffrey W. Pippin, Stuart J. Shankland, Jonathan T.C. LiuJoshua C. Vaughan

We report the use of conventional, commercially available fluorescent dyes for rapid and intense covalent labeling of proteins and carbohydrates in super-resolution (expansion) microscopy and cleared tissue microscopy. This approach, which we refer to as Fluorescent Labeling of Abundant Reactive Entities (FLARE), produces simple and robust stains that are modern equivalents of classic small-molecule histology stains. It efficiently reveals a wealth of key landmarks in cells and tissues...

Estimating the prevalence of undocumented immigrants with end-stage renal disease in the United States

Rudolph Rodriguez, Lilia Cervantes, Rajeev Raghavan

In the current political environment, the plight of undocumented immigrants in the United States (U.S.) remains perilous and uncertain, and this precarious situation is magnified for undocumented immigrants with end-stage renal disease (ESRD). For this population, access to hemodialysis varies dramatically from standard-of-care thrice-weekly hemodialysis, to the other extreme of emergency-only hemodialysis which is the practice of offering hemodialysis only after a patient meets “critically ill criteria.” Due to the exclusion from Medicare, undocumented immigrants are not included in the United States Renal Data System (USRDS), and therefore the prevalence of undocumented immigrants with ESRD in the U.S. remains unknown.

Persistent Disease Activity in Patients With Long-Standing Glomerular Disease

Elisa Delbarba, Maddalena Marasa, Pietro A. Canetta, Stacy E. Piva, Debanjana Chatterje, Byum Hee Kil, Xueru Mu, Keisha L.Gibson, Michelle A. Hladunewich, Jonathan J. Hogan, Bruce A. Julian, Jason M.Kidd, Louis-Philippe Laurin, Patrick H. Nachman, Michelle N. Rheault, Dana V. Rizk, Neil S. Sanghani, Howard Trachtman, Scott E. Wenderfer, Ali G.Gharavi, Andrew S. Bomback....J. Ashley Jefferson, et al, and on behalf of the CureGN Consortium: Katherine R. Tuttle, et al

Glomerular diseases are characterized by variable disease activity over many years. We aimed to analyze the relationship between clinical disease activity and duration of glomerular disease.

Once upon a dish: engineering multicellular systems

Kristina Haase, Benjamin S. Freedman

In February 2020, the European Molecular Biology Laboratory (EMBL) and the Institute for Bioengineering of Catalonia (IBEC) joined forces to unite researchers from all over the globe to discuss emerging topics in ‘Engineering Multicellular Systems’. As we review here, key themes that arose throughout the meeting included the ethics of organoids in developmental biology, bottom-up versus top-down models, tissue organizing principles, and the future of improving these systems to better mimic the natural world.

Caring for Nephrology Patients and Staff During the COVID-19 Pandemic: Experiences of the Northwest Kidney Centers

Ulrich, B., McNamara, L., Watnick, S., Last, F.M.

The Northwest Kidney Center (NWC) in Seattle, Washington, has been a leader in nephrology care for almost 60 years, opening the first hemodialysis unit in the United States in 1962. In February 2020, one of their patients was the first reported death from COVID-19 in the United States. On April 6, 2020, as a part of NNJ Extra - the Nephrology Nursing Journal's podcast series, Beth Ulrich, EdD, RN, FACHE, FAONL, FAAN, Editor-in-Chief of the Nephrology Nursing Journal, talked with the leaders of the Northwest Kidney Centers - Suzanne Watnick, MD, the Chief Medical Officer, and Liz McNamara, MN, RN, Vice President of Patient Care Services and the Chief Nursing Officer, who discussed dealing with the onset of COVID-19 at NWC, how their team worked together to provide care for their patients and support for their staff members, and the lessons they learned that can benefit others. Copyright© by the American Nephrology Nurses Association.

COVID-19 and Kidney Failure in the Acute Care Setting: Our Experience From Seattle

Durvasula, R., Wellington, T., McNamara, E., Watnick, S.

Northwest Kidney Centers was brought to the forefront of the COVID-19 pandemic, mandating an immediate and coordinated response. Our approach to managing the threat in our outpatient facilities has been summarized elsewhere.This editorial focuses on the acute care setting by considering 3 key questions.

Two-Phase, Generalized Case-Control Designs for the Study of Quantitative Longitudinal Outcomes

Jonathan S Schildcrout, Sebastien Haneuse, Ran Tao, Leila R Zelnick, Enrique F Schisterman, Shawn P Garbett, Nathaniel D Mercaldo, Paul J Rathouz, Patrick J Heagerty

We propose a general class of 2-phase epidemiologic study designs for quantitative, longitudinal data that are useful when phase 1 longitudinal outcome and covariate data are available but data on the exposure (e.g., a biomarker) can only be collected on a subset of subjects during phase 2. To conduct a study using a design in the class, one first summarizes the longitudinal outcomes by fitting a simple linear regression of the response on a time-varying covariate for each subject.

Kidney Function in Patients With Type 2 Diabetes After Vitamin D Supplementation—Reply

Ian H. de Boer, Leila R. Zelnick, JoAnn E. Manson

In Reply - Dr Peiris suggests that 25(OH)D concentrations, adherence, or vitamin D3 dose may have limited testing the effects of vitamin D3 on change in eGFR in our study.1 We measured serum 25(OH)D to verify adherence, confirm biomarker differences by treatment assignment, and conduct subgroup analyses. Measurements were available at baseline and 2 years for 1257 (96%) and 902 (69%) participants, respectively. At year 2, median time of blood draw was 9:40 am (interquartile range, 8:45-11:00 am), which did not differ significantly by treatment assignment.

Creating a Palliative Care Inpatient Response Plan for COVID-19—The UW Medicine Experience

Fausto, J., Hirano, L., Lam, D., Mehta, A., Mills, B., Owens, D., Perry, E., Curtis, J.R.

The coronavirus disease 2019 (COVID-19) pandemic is stressing health care systems throughout the world. Significant numbers of patients are being admitted to the hospital with severe illness, often in the setting of advanced age and underlying comorbidities. Therefore, palliative care is an important part of the response to this pandemic. The Seattle area and UW Medicine have been on the forefront of the pandemic in the U.S.