Our faculty research broadens the knowledge of kidney disease.

Serum Aldosterone Concentration, Blood Pressure, and Coronary Artery Calcium: The Multi-Ethnic Study of Atherosclerosis

Kosuke Inoue, Deena Goldwater, Matthew Allison, Teresa Seeman, Bryan R. Kestenbaum, Karol E. Watson

Aldosterone is a steroid hormone regulating fluid and electrolyte homeostasis and is known to increase the risk of atherosclerosis. In this study, we examined the associations of serum aldosterone concentrations with subclinical atherosclerosis and all-cause mortality. This study included 948 adults aged 46 to 88 years from the MESA (Multi-Ethnic Study of Atherosclerosis) with measurements of serum aldosterone and plasma renin activity and not taking antihypertensive medications.

Metabolomic Markers of Kidney Function Decline in Patients With Diabetes: Evidence From the Chronic Renal Insufficiency Cohort (CRIC) Study

Brian Kwan, Tobias Fuhrer, Jing Zhang, Manjula Darshi, Benjamin Van Espen, Daniel Montemayor, Ian H de Boer, Mirela Dobre, Chi-Yuan Hsu, Tanika N Kelly, Dominic S Raj, Panduranga S Rao, Santosh L Saraf, Julia Scialla, Sushrut S Waikar, Kumar Sharma, Loki Natarajan, CRIC Study Investigators

Biomarkers that provide reliable evidence of future diabetic kidney disease (DKD) are needed to improve disease management. In a cross-sectional study, we previously identified 13 urine metabolites that had levels reduced in DKD compared with healthy controls. We evaluated associations of these 13 metabolites with future DKD progression.

Level and Change in N-terminal Pro B-type Natriuretic Peptide and Kidney Function and Survival to Age 90

Astrid D Häberle, Mary L Biggs, Mary Cushman, Bruce M Psaty, Anne B Newman, Michael G Shlipak, John Gottdiener, Chenkai Wu, Julius M Gardin, Nisha Bansal, Michelle C Odden

Many traditional cardiovascular risk factors do not predict survival to very old age. Studies have shown associations of estimated glomerular filtration rate (eGFR) and N-terminal pro-B-type natriuretic peptide (NT-pro-BNP) with cardiovascular disease and mortality in older populations. This study aimed to evaluate the associations of the level and change in eGFR and NT-pro-BNP with longevity to age 90 years.

Open Microfluidic Coculture Reveals Paracrine Signaling From Human Kidney Epithelial Cells Promotes Kidney Specificity of Endothelial Cells

Tianzi Zhang, Daniel Lih, Ryan J Nagao, Jun Xue, Erwin Berthier, Jonathan Himmelfarb, Ying Zheng, Ashleigh B Theberge

We designed an open microfluidic platform to study the role of human kidney tubular epithelial cells in supporting EC specificity. The platform consists of two independent cell culture regions segregated with a half wall; culture media is added to connect the two culture regions at a desired timepoint, and signaling molecules can travel across the half wall (paracrine signaling).

Feature-rich covalent stains for super-resolution and cleared tissue fluorescence microscopy

Chenyi Mao, Min Yen Lee, Jing-Ru Jhan, Aaron R. Halpern, Marcus A. Woodworth, Adam K. Glaser, Tyler J. Chozinski, Leonard Shin, Jeffrey W. Pippin, Stuart J. Shankland, Jonathan T.C. LiuJoshua C. Vaughan

We report the use of conventional, commercially available fluorescent dyes for rapid and intense covalent labeling of proteins and carbohydrates in super-resolution (expansion) microscopy and cleared tissue microscopy. This approach, which we refer to as Fluorescent Labeling of Abundant Reactive Entities (FLARE), produces simple and robust stains that are modern equivalents of classic small-molecule histology stains. It efficiently reveals a wealth of key landmarks in cells and tissues...

Estimating the prevalence of undocumented immigrants with end-stage renal disease in the United States

Rudolph Rodriguez, Lilia Cervantes, Rajeev Raghavan

In the current political environment, the plight of undocumented immigrants in the United States (U.S.) remains perilous and uncertain, and this precarious situation is magnified for undocumented immigrants with end-stage renal disease (ESRD). For this population, access to hemodialysis varies dramatically from standard-of-care thrice-weekly hemodialysis, to the other extreme of emergency-only hemodialysis which is the practice of offering hemodialysis only after a patient meets “critically ill criteria.” Due to the exclusion from Medicare, undocumented immigrants are not included in the United States Renal Data System (USRDS), and therefore the prevalence of undocumented immigrants with ESRD in the U.S. remains unknown.

Persistent Disease Activity in Patients With Long-Standing Glomerular Disease

Elisa Delbarba, Maddalena Marasa, Pietro A. Canetta, Stacy E. Piva, Debanjana Chatterje, Byum Hee Kil, Xueru Mu, Keisha L.Gibson, Michelle A. Hladunewich, Jonathan J. Hogan, Bruce A. Julian, Jason M.Kidd, Louis-Philippe Laurin, Patrick H. Nachman, Michelle N. Rheault, Dana V. Rizk, Neil S. Sanghani, Howard Trachtman, Scott E. Wenderfer, Ali G.Gharavi, Andrew S. Bomback....J. Ashley Jefferson, et al, and on behalf of the CureGN Consortium: Katherine R. Tuttle, et al

Glomerular diseases are characterized by variable disease activity over many years. We aimed to analyze the relationship between clinical disease activity and duration of glomerular disease.

Once upon a dish: engineering multicellular systems

Kristina Haase, Benjamin S. Freedman

In February 2020, the European Molecular Biology Laboratory (EMBL) and the Institute for Bioengineering of Catalonia (IBEC) joined forces to unite researchers from all over the globe to discuss emerging topics in ‘Engineering Multicellular Systems’. As we review here, key themes that arose throughout the meeting included the ethics of organoids in developmental biology, bottom-up versus top-down models, tissue organizing principles, and the future of improving these systems to better mimic the natural world.

Caring for Nephrology Patients and Staff During the COVID-19 Pandemic: Experiences of the Northwest Kidney Centers

Ulrich, B., McNamara, L., Watnick, S., Last, F.M.

The Northwest Kidney Center (NWC) in Seattle, Washington, has been a leader in nephrology care for almost 60 years, opening the first hemodialysis unit in the United States in 1962. In February 2020, one of their patients was the first reported death from COVID-19 in the United States. On April 6, 2020, as a part of NNJ Extra - the Nephrology Nursing Journal's podcast series, Beth Ulrich, EdD, RN, FACHE, FAONL, FAAN, Editor-in-Chief of the Nephrology Nursing Journal, talked with the leaders of the Northwest Kidney Centers - Suzanne Watnick, MD, the Chief Medical Officer, and Liz McNamara, MN, RN, Vice President of Patient Care Services and the Chief Nursing Officer, who discussed dealing with the onset of COVID-19 at NWC, how their team worked together to provide care for their patients and support for their staff members, and the lessons they learned that can benefit others. Copyright© by the American Nephrology Nurses Association.

COVID-19 and Kidney Failure in the Acute Care Setting: Our Experience From Seattle

Durvasula, R., Wellington, T., McNamara, E., Watnick, S.

Northwest Kidney Centers was brought to the forefront of the COVID-19 pandemic, mandating an immediate and coordinated response. Our approach to managing the threat in our outpatient facilities has been summarized elsewhere.This editorial focuses on the acute care setting by considering 3 key questions.